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. 2021 May 11;12(3):857–871. doi: 10.1016/j.jcmgh.2021.05.006

Figure 4.

Figure 4

AMPK is required for Insig-1 induction in response to NaB treatment. (A–C) NaB increases the expression levels of phosphorylated AMPK in mice fed with HFD. (A) Representative immunohistochemical staining of the liver sections with phosphorylated AMPK (pAMPK) and phosphorylated ACC (pACC) antibodies (scale bar = 50 μm). (B) Immunoblots were performed, and (C) the band intensity of pAMPK and pACC was quantified by densitometry (n = 6). (D) AMPK is required for Insig-1 induction in response to NaB treatment. AMPK+/+ or AMPKα1/α2 double knockout (AMPK−/−) mouse primary hepatocytes were infected with adenoviruses encoding Insig-1 (Ad-Insig-1) or green fluorescent protein (GFP) for 24 hours, followed by treatment with 5 mM or 10 mM NaB for 24 hours. The data are presented as the mean ± SEM, unpaired 2-tailed Student’s t test, n = 6. ∗P < .05 vs mice fed with chow diet; #P < .05 vs mice fed with HFD.