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. 2021 Jul 27;13:e00180. doi: 10.1016/j.mec.2021.e00180

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© 2021 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 2 — Transcript analysis of RZS1 and BAS expression in transgenic tobacco (T₂generation). (A) Expression construct of RZS1 and BAS used in this study. (B) The expression levels of RiRZS1 in RZS1-BAS overexpressing transgenic tobacco leaves. (C) The expression levels of RpBAS in RZS1-BAS-overexpressing transgenic tobacco leaves. Expression levels were examined using quantitative RT-PCR and normalized to those of the internal reference gene Elongation Factor 1 alpha (EF1a). Significant differences were identified using one way ANOVA with Fisher's least significance differences test (P < 0.05). N.D., not detected. _pro35 S, CaMV35S promoter; AtADH 5′-UTR, five prime untranslated region of A. thaliana alcohol dehydrogenase; RiRZS1, Rubus idaeus raspberry ketone/zingerone synthase 1; RpBAS, Rheum palmatum benzalacetone synthase; _terHSP, terminator of A. thaliana heat shock protein.