Mechanical properties. Young’s modulus (elasticity) in kPa and standard deviation (sd) of
S. epidermidis
1585 biofilms, calculated from AFM nanoindentation. Biofilms in which PIA production was induced [
S. epidermidis
1585pTXicaADBC grown in TSB and
S. epidermidis
1585pTXicaADBC grown in BHI with 10 % (v/v) human plasma (HP)] were less stiff than biofilms in which only Embp was induced [(
S. epidermidis
1585Pxyl/tetembp grown in BHI with 10 % (v/v) plasma] [Welch’s t-test, ±sd, t(df)]: 0.876±0.521, n=3, P<0.05; and 1.263±0.403, n=3, P<0.05; compared with 2.616±0.243, n=3. Each sample (n) represents 1 biological replica with 5 measurement points and 10 force curves at each point.