Table 2.
Comparison of single nucleotide polymorphism (SNP) detection for the MG+parC(beta2) assay compared to Sanger sequencing*
|
Sanger sequencing |
||||
|---|---|---|---|---|
|
|
|
Mutant |
Wild-type |
Total |
|
MG+parC(beta2) |
Mutant |
54† |
1‡ |
55 |
|
Wild-type |
1§ |
149 |
150 |
|
|
|
Total |
55 |
150 |
|
*Both baseline and test of cure samples are included.
†The 40 G248T/S83I SNPs (all detected by assay), 13 G259A/D87N mutations (12/13 detected by assay), 2 G248A/S83N mutations (all detected by assay). SNPs in the parC quinolone-resistance-determinant region not targeted by this assay include A260G/D87G, A247T/S83C.
‡Determined to be WT by sequencing, but G259A/D87N by MG+parC(beta2).
§Determined to be G259A/D87N by sequencing.