Fig. 1.

UGP2 is critical for PDAC cell survival and proliferation in vitro and in vivo. (A) Colony counts of Panc1 and Suit2 cells grown in two-dimensional culture conditions for 10 d; n = 3; ***P < 0.001, ****P < 0.0001. (B) Relative viability of Panc1 and Suit2 cells grown in three-dimensional culture conditions for 14 d, measured by CellTiter-Glo; n = 4; ****P < 0.0001. (C) Tumor volumes of MiaPaca2 and Suit2 cells with shUGP2 or empty vector control xenografted on opposite flanks of nude mice; n = 5; *P < 0.05. Error bars represent SEM. (D) Representative immunohistochemical staining of paired opposite-flank tumors with shUGP2 or vector control at end point, stained as indicated. H&E, hematoxylin and eosin. (Scale bars, 50 μm.)