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. 2021 Jul 30;118(31):e2103592118. doi: 10.1073/pnas.2103592118

Fig. 2.

Fig. 2.

UGP2 is a target gene of YAP. (A) Representative tissue microarray images stained for YAP or UGP2. B-9, B-15, and G-11 indicate sample codes. (B) Scoring of tissue microarrays containing 78 pancreatic ductal adenocarcinoma samples immunohistochemically stained for YAP or UGP2. +, ++, and +++ represent combined scoring of automated area × intensity measurements. ****P < 0.0001 by χ2 test. (C) qPCR for UGP2 mRNA in Panc1, MiaPaca2, and Suit2 cells with shYAP or empty vector. ***P < 0.001, error bars represent SEM. (D) Immunoblot on lysates from Panc1, MiaPaca2, and Suit2 cells stably expressing shYAP or empty vector, probed as indicated. (E) ChIP-seq for YAP1 and TEAD4 at the UGP2 locus. (F) Targeted PCR using two sets of primers (p1 and p2) in the UGP2 promoter region of Panc1 cells after cross-linking and pull-down assays using antibodies against either YAP, TEAD4, or control immunoglobulin G (IgG). Fold enrichment is percent input target gene/percent input actin. *P < 0.05, **P < 0.01, error bars represent SEM.