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. 2021 Jul 26;118(31):e2100862118. doi: 10.1073/pnas.2100862118

Fig. 4.

Fig. 4.

Increased ECM in the CNS from OL-specific Thap1 deletion. (A) Representative image (scale bar, 1 mm) demonstrating C-4S GAG (2H6) staining for control (Thap1flx/+) (Top) and O-CKO (Thap1flx/−;Olig2-Cre+) (Bottom) mouse P80 tissue. Dashed boxes highlight the CC, AC, and cortex, which are analyzed in control and O-CKO insets. (B) Representative image (scale bar, 100 μm) of the cortical thickness spanning all layers, from CC to pial surface, stained for 2H6. (C–F) Representative images (scale bar, 100 μm) and corresponding quantification of 2H6 staining in CC (C and D) (control = 114.0 arbitrary units (AU) /mm2 ± 31.26; O-CKO = 362.4 AU /mm2 ± 47.48; t test; t(8) = 3.86, P = 0.0048; n = 3 for control and 4 for O-CKO) and AC (E and F) (control = 147.7 AU /mm2 ± 16.12; O-CKO = 485.5 AU /mm2 ± 23.84; t test; t(5) = 12.24, P < 0.0001; (n = 3 for control and 4 for O-CKO). (G) Representative images (scale bar, 100 μm) of 2H6-stained cortex. Arrow heads indicate DACS structures formed in control P80 tissue, which are not observed in O-CKO tissue because of the increased overall expression.