Figure 2.

Identification of 16 compounds reversing XP-C photo-sensitivity upon UVB irradiation. Prestwick library of chemical drugs was screened on XP-C cells. The cells were treated with the library for 24 h, then further on, irradiated. Post Irradiation, the cells were then incubated with the library for an additional 24 h prior to having their viability assessed 24 h post UV via the addition of Presto Blue reagent and the recording of the fluorescent values. Based on two different normalization criteria, control based % of activity and non-control based RZscore, 16 compounds were selected manifesting >25% increase or >2.5 value for both % of activity and RZscore respectively. % Activity was calculated by normalizing the obtained values between the interval of 100% donated to non-irradiated DMSO treated cells and 0% that donated irradiated DMSO treated cells. RZscore was measured by normalizing the fluorescence values to the median and median absolute deviation. RZscore: Robust Z score.