Figure 4.

Keratin profiles of differentially expressed keratin genes in non-label retaining prostasphere cells. (A) Single-cell RNA-seq of the sorted CFSE^Low prostasphere cells with subsequent keratin gene heatmap clustering reveals three subpopulations of progenitor cells: cluster IV (right), cluster V (middle), and cluster VI (left). Cluster IV is enriched in KRT14, 5, 16, and 6, indicating a basal progenitor cell lineage. Clusters V and VI are enriched in KRT8, 18, and 10, indicating a luminal cell lineage. (B) TSCAN analysis of KRT8 and KRT18 in CFSE^Low cells suggests a relationship between cluster V (2) and VI (1), but not cluster IV (3). Relative gene expression levels are noted numerically for each cluster.