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Keratin profiling in tumor spheroids using single-cell RNA-seq identifies cancer stem-like cells. (A,B) Uniform manifold approximation and projection (UMAP) clustering defined five clusters in cancer cells, identifying cluster 2 as cancer stem-like cells enriched for stemness markers, including KRT13. (C) Keratin gene profiling by heatmap and (D) dot-plot analyses identified a group of keratin genes enriched in the cluster 2 cancer stem-like cells.
