Table 1.
Gene expression in PBMCs at 6 h culture.
| Gene Expression (Relative to Housekeeping Gene HPRT1) | ||||||||
|---|---|---|---|---|---|---|---|---|
| Sample, N = 7 donors | TNFA | IL1B | IL6 | CXCL8 | ||||
| Control | 3.1 | (1.2–4.8) | 43.2 | (34.4–55.4) | 0.07 | (0.03–0.10) | 75.6 | (46.2–95.3) |
| LPS | 31.5 | (38.4–59.9) | 559.2 | (452.8–686.0) | 63.0 | (40.8–105.7) | 965.5 | (852.4–1394.4) |
| LPS + AAT | 28.1 | (8.3–63.0) | 511.6 | (490.8–625.3) | 62.8 | (43.7–95.6) | 986.6 | (809.5–1126.6) |
| LPS + oxAAT | 28.6 | (9.4–34.6) | 574.2 | (447.3–733.7) | 47.7 | (40.7–83.4) | 1077.9 | (833.5–1247.1) |
| LPS + recAAT | 14.6 | (8.0–32.7) | 637.3 | (489.4–725.4) | 50.3 | (35.5–68.0) | 1208.9 | (1001.2–1323.7) |
| LPS + Spike | 33.7 | (24.4–163.1) | 853.2 | (729.1–903.9) | 110.4 | (83.8–127.2) | 1540.2 | (1248.2–1983.4) |
| LPS + Spike + AAT | 34.5 | (25.7–109.7) | 751.5 | (639.2–903.6) | 102.6 | (56.5–112.7) | 1735.2 | (1319.5–1841.3) |
| LPS + Spike + oxAAT | 33.3 | (20.7–129.1) | 668.4 | (517.2–868.8) | 104.6 | (49.2–124.5) | 1610.6 | (846.7–2190.2) |
| LPS + Spike + recAAT | 16.8 | (13.4–38.8) * | 472.5 | (320–754.1) ** | 49.7 | (32.3–73.8) | 1183.9 | (1012.7–1597.4) |
Data presented as median (range), bold shows significant difference between LPS/spike and LPS/spike + recAAT, * p < 0.05, ** p < 0.01. All measurements were performed in duplicates, with samples from seven independent donors using 2.5 × 106 cells per experimental condition.