Figure 3.

The concentrations of the IL-17 (A), IL-10 (B), IFN-γ (C), IL-1β (D), and IL-8 (E) cytokines in comparison with the challenge experimental NE study obtained through ELISA. At 19 days of age, the chickens were infected for 4 continuous days through oral administration of broth cultures of C. perfringens. On the second day of C. perfringens infection, the chicks were orally administrated the recommended dose of the coccidial vaccine 10 times. The treatment was administrated in drinking water on day 22 and continued for 5 days. Sera were collected at day 27 postchallenge. The values are means ± S.E. Different letters represent statistically significant differences with a p-value less than 0.05.