Figure 1.

Ca²⁺ signals of white adipocytes due to the application of calcium-free (Ca²⁺-free) medium. (A) The Ca²⁺-free medium induces the generation of various Ca²⁺ responses: transient in 32% of adipocytes, Ca²⁺ oscillations without a lag-phase in 45% of adipocytes and Ca²⁺ oscillations with a lag-phase in 23% of adipocytes. Recovery of the extracellular Ca²⁺ concentration to a standard level (+1.2 mM Ca²⁺) does not induce changes in the Ca²⁺ dynamics of adipocytes. Averaged Ca²⁺ signals obtained from 15 adipocytes in one experiment for each curve (n = 15) are shown. The experiment was performed in 6 repetitions (n = 6) on three separate cell cultures. (B) Repeated application of Ca²⁺-free medium to the culture of white adipocytes (after a 30-min period of the cell culture recovery) leads to the generation of Ca²⁺ signals similar in shape and amplitude. The Ca²⁺ signals of single adipocytes are presented.