Table 1.

Dam body composition and pregnancy outcomes. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. Con, ^# p < 0.05, ^## p < 0.01, ^### p < 0.001 vs. Ob using one-way ANOVA with Tukey’s multiple comparison test, Kruskal–Wallis test for nonparametric data (gestation length and litter size) or ANOVA performed on log-transformed data (PN2 weight). E15.5 = embryonic day 15.5. ns = not significant. PN2 = postnatal day 2.

	Con	Ob	Ob-Met	p-Value
Dams at E15.5	n = 15	n = 13	n = 6–7
Body weight (g)	36.0 ± 0.7	49.0 ± 0.8 ***	44.9 ± 1.2 ***,#	<0.0001
Lean mass (g)	23.9 ± 0.7	24.7 ± 0.6	23.9 ± 0.6	ns
Fat mass (g)	4.5 ± 0.3	15.6 ± 0.6 ***	12.4± 0.6 ***,###	<0.0001
Fat mass (%)	12.5 ± 0.7	31.7 ± 0.9 ***	27.6 ± 0.9 ***,###	<0.0001
Pregnancy outcome	n = 24–31	n = 30–33	n = 20–26
Gestation length (d)	20 [19, 20]	20 [21, 22]	21 [20.3–21] ***,##	<0.0001
Litter size (no pups)	7.0 [6.0–9.0]	6.0 [5.0–7.0] **	6.0 [5.0–7.0] *	0.0015
PN2 weight (g/pup)	1.9 ± 0.04	1.6 ± 0.04 ****	1.7 ± 0.04 ***	<0.0001