Table 1.
Intra-day and inter-day precision and accuracy of LC/MS/MS method for the determination of erinacine A.
| Intra-Day | Inter-Day | |||||
|---|---|---|---|---|---|---|
| Theoretical Conc. | Observed Conc. | Precision | Accuracy | Observed Conc. | Precision | Accuracy |
| (ng/mL) | (ng/mL) | (% CV) | (% bias) | (ng/mL) | (% CV) | (% bias) |
| 5 | 4.27 ± 0.37 | 8.79 | −14.70 | 4.08 ± 0.52 | 9.34 | −9.66 |
| 10 | 12.85 ± 0.68 | 8.23 | 14.00 | 11.40 ± 0.94 | 4.04 | 10.33 |
| 20 | 17.48 ± 0.61 | 3.50 | −12.58 | 16.99 ± 0.98 | 5.76 | −15.00 |
| 50 | 52.55 ± 1.04 | 1.98 | 5.10 | 49.69 ± 1.28 | 2.59 | −0.62 |
| 100 | 104.50 ± 1.76 | 1.68 | 4.50 | 103.49 ± 12.03 | 11.63 | 3.49 |
| 200 | 207.83 ± 2.40 | 1.16 | 3.92 | 178.67 ± 9.19 | 5.14 | −10.67 |
| 500 | 479.17 ± 8.84 | 1.85 | −4.17 | 466.33 ± 31.11 | 6.67 | −6.73 |
Data expressed as means ± SD (n = 6). CV (%) = (standard deviation/mean) × 100%. bias (%) = [(measured concentration − theoretical concentration)/theoretical concentration] × 100%.