Figure 3.

Effect of culture medium alone (CM), peritoneal fluid from control woman (Control PF) or peritoneal fluid from woman with endometriosis (ENDO PF) on generation of CD25^high and CD25^high FOXP3⁺ Treg cells in cultures of CD4⁺ T cells unstimulated or stimulated with CD3/CD28 beads and IL-2. (A) Gating strategy and a representative flow cytometry analysis showing identification of the respective CD25^high and CD25^high FOXP3⁺ T cell subpopulations in CD4⁺ T cells under different culture conditions. (B) Proportions of CD25^high T cells and (C) CD25^high FOXP3⁺ Treg cells in population of unstimulated or CD3/CD28 beads+IL-2-stimulated CD4⁺ T cells. The results are shown as scatter dot plots with a median and interquartile range. Statistical significance was computed by paired (Friedman’s test) or unpaired (Kruskal–Wallis test) non-parametric ANOVA followed by a post hoc test.