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. 2021 Jul 29;22(15):8134. doi: 10.3390/ijms22158134

Figure 5.

Figure 5

Effect of culture medium alone (CM), peritoneal fluid from control woman (Control PF) or peritoneal fluid from woman with endometriosis (ENDO PF) on the NK cell cytotoxic activity of cultured PBMC. (A) The representative flow cytometry analysis showing the controls for the NK assay. Shown are the fluorescence-negative effector cells (PBMC), green fluorescence (FITC) labeled K562 target cells and spontaneously dying K562 cells (red fluorescence, PE). Spontaneous death of target cells was determined in cultures without effector cells. (B) An example of identification of target K562 cells killed by NK cells from the PBMC population (red fluorescence). (C) Relative cell mediated cytotoxicity of untreated and peritoneal fluid-treated PBMC against K562 cells. The results are expressed as an index of specific cytotoxicity of peritoneal fluid-treated PBMC relative to untreated control PBMC. Each bar represents mean ± SD from 4 independent experiments. Statistical significance was computed by paired non-parametric ANOVA (Friedman’s test) followed by a post hoc test.