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. 2021 Jul 27;10(15):3305. doi: 10.3390/jcm10153305

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Flow cytometric gating strategy of AIM assay to identify specific T-helper cells (THC), and central memory T-cell (Tcm) and effector memory T-cell (Tem) subsets from a participant included in the study: (A) lymphocytes (Gate P1) were gated using forward and side scatter; (B) gated on P1: THC (Gate P2) were defined by co-expression of CD3 and CD4; (D) gated on P2: specific THC (Gate P3) were analyzed as CD25 high/CD134 high double-positive cell population after stimulation with SARS-CoV-2; (C) borders of Gate P3 were defined by means of unstimulated THC population; (F) gated on P3: specific THC were further divided into Tcm and Tem; (E) borders between Tcm, Tem and naïve T-cells were defined by unstimulated THC.