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Inhibitory effect of HDE on the MAPKs in LPS-stimulated BV2 cells. (A–D) Western blot analysis on the p38, ERK1/2, and JNK phosphorylation levels in the BV2 cells. The cells were pretreated with HDE 2–10 µg/mL for 2 h prior to LPS (1 µg/mL) exposure for 24 h. (E,F) Western blot analysis on ph-p65 and protein expression in the BV2 cells when pretreated with HDE prior to LPS exposure. β-actin was used as the housekeeping protein.
