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. 2021 Jul 22;26(15):4425. doi: 10.3390/molecules26154425

Figure 1.

Figure 1

(a) Fluorescence spectra changes of Py-SiRh-NTR (10 μM) treated with the increasing concentrations of NTR in the presence of 200 μM NADH for 180 min at 37 °C in PBS λex = 645 nm. (b) The linear correlation between the fluorescence intensity of Py-SiRh-NTR and the concentration of NTR. (c) The absorption spectra of Py-SiRh-NTR (10 μM) in the absence of NTR and in the presence of NTR. (d) Time-dependent fluorescent response spectra of Py-SiRh-NTR (10 μM) in the presence of varied concentrations of NTR (from bottom to top): 0, 1.0, 3.0, 3.5 μg/mL.