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SDS-PAGE electrophoresis of prokaryotic expression of pET28a-UGT84A77. M, Protein Marker; lane 1, pET28a without isopropyl-β-d-thiogalactopyranoside (IPTG); lane 2, pET28a, induced by 0.2 mM IPTG; lane 3, pET28a-UGT84A77 without IPTG; lane 4, pET28a-UGT84A77, induced by 0.2 mM IPTG; the arrow indicates the expressed fusion protein, approximately 57 kDa.
