Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 31;26(15):4650. doi: 10.3390/molecules26154650

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

SDS-PAGE determination of purified prokaryotic expression protein pET28a-UGT84A77. M, protein marker; lane 1 indicates protein expression not induced by IPTG; lane 2 indicates total protein induced by IPTG; lane 3 indicates total soluble protein in supernatant; lane 4 indicates insoluble protein in pellet; lane 5 indicates effluent after flowing through resin; lane 6 indicates washing buffer after flowing through resin; lanes 7–15 indicate the proteins eluted by 40, 60, 80, 100, 120, 140, 160, 180, and 200 mM imidazole eluents, respectively; the underline indicates the purified objective protein of pET28a-UGT84A77.