Table 2.
The regression equations with R2 value, linearity, LOD, LOQ, precision, stability, repeatability, and accuracy.
| Analyte | Equation of Calibration Curve |
Determination Coefficient (R2) |
Linear Range (μg/mL) |
LOD (μg/mL) |
LOQ (μg/mL) |
Precision RSD (%), n = 3 |
Stability RSD (%) |
Repeatability RSD (%) |
Accuracy | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Intra-Day AUC |
Inter-Day AUC |
Mean % Recovery | Mean RSD (%) | ||||||||
| Caffeic acid | y = 577,684x + 504.35 | 0.9801 | 0.05–0.50 | 0.017 | 0.05 | 1.74 | 1.58 | 1.1 | 1.3 | 100.48 | 1.25 |
| p-Coumaric acid | y = 2 × 106x + 98.8 | 0.9999 | 0.01–0.05 | 0.003 | 0.01 | 1.92 | 1.85 | 1.5 | 0.5 | 100.37 | 1.69 |
| Ferulic acid | y = 730,425x + 8016.8 | 0.9811 | 0.001–0.05 | 0.0003 | 0.001 | 2.23 | 2.10 | 0.8 | 1.0 | 100.57 | 1.97 |
| Quercetin | y = 1 × 106x − 14,970 | 0.9651 | 0.01–0.30 | 0.003 | 0.01 | 1.66 | 1.52 | 0.9 | 1.6 | 100.25 | 1.01 |
| Cinnamic acid | y = 1 × 106x − 176.2 | 0.9997 | 0.01–0.30 | 0.003 | 0.01 | 4.31 | 4.19 | 0.8 | 0.7 | 100.60 | 1.20 |
| Naringenin | y = 9 × 106x − 12,307 | 0.9996 | 0.01–0.10 | 0.003 | 0.01 | 6.50 | 6.37 | 1.0 | 0.8 | 100.39 | 1.10 |
| Pinocembrine | y = 1 × 107x − 1492.3 | 0.9998 | 0.01–1.00 | 0.003 | 0.01 | 5.19 | 4.99 | 1.2 | 1.3 | 100.51 | 1.65 |
| Chrysin | y = 8 × 106x − 1982.3 | 0.9999 | 0.005–0.05 | 0.0016 | 0.005 | 3.83 | 3.72 | 0.7 | 1.9 | 100.59 | 1.43 |
| Galangin | y = 4 × 106x + 6009.3 | 0.9947 | 0.01–0.30 | 0.003 | 0.01 | 4.55 | 4.47 | 1.2 | 1.4 | 100.44 | 1.23 |
LOD: limit of detection; LOQ: limit of quantification; RSD: relative standard deviation; AUC: area under curve.