Figure 4.

(A) The effect of the incubation of SW620 cells with the vehicle (0 µM), 10 and 30 µM of DW-8, followed by DAPI, on cell nuclear morphology. The nuclei of the cells incubated with the vehicle were circular and intact, whereas the nuclei of cells incubated with 10 and 30 µM of DW-8 were shrunken and fragmented. (B) The effect of vehicle (0 µM DW-8), 10 µM of DW-8 and 0.5 µM of paclitaxel (PTX) on the fluorescence level of the compound DCFDA, due to the biotransformation of H₂DCFDA by cellular esterases. The level of green fluorescence and thus, the levels of ROS, produced by DW-8 and paclitaxel, was significantly greater (p-value < 0.0001) than the negative control. The data represent the means ± SD of two independent experiments.