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. 2021 Jul 26;22(15):7988. doi: 10.3390/ijms22157988

Figure 6.

Figure 6

PBMCs, NK cells, and NK-T cells isolated from healthy donors were treated with high glucose concentration for 48 h. Glucose were added every 24 h at 5.5 mM, 25 mM, and 45 mM concentrations. Total PBMCs (A) had a significant increase in leptin secretion after 45 mM glucose vs. both 5.5 mM glucose and 25 mM glucose; on the contrary, PBMCs treated with 25 mM glucose did not have a significant increase in leptin secretion (p = 0.0587). Isolated NK cells (B) displayed a significant increase in leptin secretion in response to 25 mM glucose and 45 mM glucose stimulation compared to 5.5 mM. NK-T cells (C) showed a significant increase in leptin secretion in the same experimental conditions as NK cells, with the highest levels in response to glucose 45 mM (~46 pg/mL). Data are expressed as mean ± SEM for three healthy donors and were analyzed with a Wilcoxon–Mann–Whitney U Test (** p < 0.005, *** p < 0.0005). PBMC: peripheral blood mononuclear cells; NK: Natural Killer; NK-T: Natural Killer-T; glu: glucose.