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. 2021 Jul 26;22(15):7948. doi: 10.3390/ijms22157948

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Analysis of the apoptosis rate in (a) MDA-MB-231 and (b) SK-BR-3 cancer cell lines after incubation with different samples. Q1 (necrotic cells), Q2 (late apoptosis), Q2 + Q3 (early apoptosis), and Q4 (alive cells). Treatments of range concentrations (IC50 concentration) of nano- and free drugs were applied to the cells for 48 h. Cell cycle analysis for (c) MDA-MB-231 and (d) SK-BR-3 cancer cell lines after incubation with different samples. S1: letrozole; S2: curcumin; S3: mixture of letrozole and curcumin; S4: NiCoFe₂O₄@L-Silica@Niosome; S5: NiCoFe₂O₄@L-Silica@L-Niosome; S6: NiCoFe₂O₄@L-Silica@C-Niosome. Control: untreated cells.