Figure 5.

Flow cytometry analysis of the cellular uptake of composite materials. Cells were incubated for 48 h in cell culture medium containing various microgels that contain the expression vector pcDNA EXP-mKate. (a) Control in which HeLa cells were transfected with the vector, using Lipofectamine 2000 but no composite materials. (b) HeLa cells incubated with the vector-containing SC50 microgel. The dot plot displays single and live cells previously gated based on SSC-A vs. SSC-W signals and negative for DAPI. The bars in (c) show the comparison of S100 microgel uptake by various different cell lines. (d) Comparison of the uptake of different composite materials by HeLa cells. Standard deviation in technical triplicates for (c,d) was less than 0.3% for >10,000 cells counted per run.