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. 2021 Jul 27;22(15):8024. doi: 10.3390/ijms22158024

Figure 6.

Figure 6

Transactivation of the Tal1 promoter by EKLF. (A) Linear maps of the wild type and three mutant fragments, in which the CACCC box E1, E2, or E3 was mutated, and used for construction of the reporter plasmids pTal1-Luc, pTal1(Mut E1)-Luc, pTal1(Mut E2)-Luc, and pTal1(Mut E3)-Luc, respectively. (B) Luciferase reporter assay of the Tal1 promoter in 293 cells. The dose-dependence of the luciferase (Luc) activity on the amount (ug) of pFlag-EKLF used in co-transfection is shown in the bar diagram. * p < 0.05, *** p < 0.001 by t test. Error bars: STD. The elevated levels of the exogenous Flag-EKLF upon co-transfection with increased amounts of the pFlag-EKLF plasmid were validated by immunoblotting.