Figure 4.

17,18-EEQ or 19,20-EDP combined with t-TUCB delivered via mini osmotic pump decreased the lipid accumulation and regulated thermogenic gene expression in the iBAT of diet-induced obesity. After termination, the iBAT tissue slides from mice were stained with hematoxylin and eosin (H&E; A), and the % area occupied by lipid from 2–3 fields per slide per mouse (n = 5) were analyzed using Image J software (B). Scale bars = 100 µm. Protein expression of PGC1α, UCP1, and the loading control ERK in the iBAT of mice in the control, T, T + EDP, and T + EEQ group (C), and their densitometry (D) are shown; Bar graphs show the normalized densitometry for PGC1α/ERK1/2 and UCP1/ERK1/2. Data = the mean ± SEM (n = 5–6). *, **, and *** = p < 0.05, p < 0.01, and p < 0.001 compared to the controls, respectively.