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. 2021 Aug 3;22(15):8345. doi: 10.3390/ijms22158345

Figure 2.

Figure 2

Amniotic epithelial cells expressed a functional RAGE axis. (A) RNA expressions of RAGE, TIRAP, Myd88, and Diaphanous-1 were detected by RT-PCR in pAECS. Negative controls were performed in the absence of a cDNA template. (B) The RAGE receptor and its adaptors, Diaphanous-1, MyD88, and TIRAP (green staining, Alexa488), were detected by immunocytochemistry on primary amniocytes (pAECs). Nuclei were counterstained with Hoechst (blue). Scales bars: 50 µM (magnification ×200). Negative control was realized without primary antibody hybridization.