. 2021 Aug 3;22(15):8345. doi: 10.3390/ijms22158345

Table 1.

Forward and reverse primer sequences used for RT-PCR and RT-qPCR amplification of human genes.

Gene	Sequence 5′-3′ (F: Forward, r: Reverse)	Product Size (bp)	Annealing Temperature (°C)
hsRAGE	F: TGTGCTGATCCTCCCTGAGA	139	61
hsRAGE	R: TGCAGTTGGCCCCTCCTCG	139	61
hs36B4	F: AGGCTTTAGGTATCACCACT	219	61
hs36B4	R: GCAGAGTTTCCTCTGTGATA	219	61
hsRSP17	F: TGCGAGGAGATCGCCATTATC	169	61
hsRSP17	R: AAGGCTGAGACCTCAGGAAC	169	61
hsMyD88	F: GCAGGAGGAGGCTGAGAAGC	177	66
hsMyD88	R: CGGATCATCTCCTGCACAAACT	177	66
hsTIRAP	F: AAGTACCAGATGCTGCAGGCC	200	66
hsTIRAP	R: AGTGTCAACTGAGTGTCTGCAG	200	66
hsDia-1	F: AGAGCCACACTTCCTTTCCATC	167	66
hsDia-1	R: TCAATCTCAATCTGGAGGTGCC	167	66
hsIL6	F: AATGAGGAGACTTGCCTGGTG	143	61
hsIL6	R: AGGAACTGGATCAGGACTTTTG	143	61
hsIL8	F: TGATTTCTGCAGCTCTGTGTG	154	61
hsIL8	R: TCTGTGTTGGCGCAGTGTGG	154	61
hsIL1β	F: AATCTCCGACCACCACTACAG	174	62
hsIL1β	R: TCCCATGTGTCGAAGAAGATAG	174	62
hsMMP9	F: ATTGACGACGCCTTTGCCCG	201	61
hsMMP9	R: ATGGGCGTCTCCCTGAATGC	201	61
hsMMP2	F: AGCTCATCGCAGATGCCTGG	199	61
hsMMP2	R: AAGGGCCTGTGGGAGCCAG	199	61