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. 2021 Aug 2;22(15):8300. doi: 10.3390/ijms22158300

Figure 6.

Figure 6

ALOX12 enhances intracellular ROS and lipid peroxidation. Cells were treated with 2.5 µM HYDROP after 50 µM H2O2 treatment for 2 h (A–D) to analyze the effect of ALOX12 on intracellular ROS generation. Lipid peroxidation was detected using an HNE antibody after 50 µM H2O2 treatment. (A–E) Intracellular H2O2 visualized by HYDROP (F–J) Lipid peroxidation visualized by HNE antibody. (A,F) HeLa CRR cells siN.C. treatment. (B,G) HeLa CRR cells with ALOX12 overexpression. (C,H) SAS CRR cells siN.C. treatment. (D,I) SAS CRR cells with ALOX12 overexpression. (E) Relative HYDROP intensity (HeLa CRR N.C.: n = 144, HeLa CRR simiR-7-5p: n = 45, SAS CRR N.C.: n = 122, SAS CRR simiR-7-5p: n = 152). (J) Relative HNE intensity (HeLa CRR N.C.: n = 58, HeLa CRR simiR-7-5p: n = 83, SAS CRR N.C.: n = 57, SAS CRR simiR-7-5p: n = 87). **: p < 0.01 using Student’s t-test. ALOX12 significantly enhances intracellular ROS and lipid peroxidation after H2O2 treatment.