Figure 1.

N-palmitoyl serinol (PS)-mediated increase in overall ceramide content in human epidermal keratinocytes (KC). HaCaT KC were incubated with PS (25 μM or as indicated) for up to 24 h. The molecular structure of PS (A) and cell viability measured by the WST-1 assay (B). Total ceramide content was assessed by the LC-MS/MS system (C). Statistical significance was calculated using the unpaired Student’s t-test, and significance was defined as * p < 0.01 vs. vehicle control (or untreated control).