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An IL-4-induced decrease in ceramide content was stimulated in cells following N-palmitoyl serinol (PS) treatment. HaCaT KC were pretreated with IL-4 (50 ng/mL) for 20 h, followed by incubation with exogenous PS (25 μM) for 4 h. Total ceramide content was assessed by the LC-MS/MS system. Statistical significance was calculated using the unpaired Student’s t-test, and significance was defined as * p < 0.01 vs. vehicle control (or untreated control).
