Figure 3.

N-palmitoyl serinol (PS)-mediated alteration in activities of key enzymes responsible for ceramide synthesis. HaCaT KC were pretreated with IL-4 (50 ng/mL) for 20 h, followed by incubation with exogenous PS (25 μM) for 4 h. Serine palmitoyltransferase (SPT) (A), ceramide synthase (CerS) 2 (B), CerS3 (C), neutral sphingomyelinase (SMase) (D), or acidic sphingomyelinase (SMase) (E) were measured by the LC-MS/MS system. Statistical significance was calculated using the unpaired Student’s t-test, and significance was defined as * p < 0.01 vs. vehicle control (or untreated control).