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. 2021 Jul 26;22(15):7951. doi: 10.3390/ijms22157951

Figure 2.

Figure 2

PRMT1 deficiency leads to the reduction of global asymmetric dimethylation as well as asymmetrical dimethylation on arginine-3 of histone H4 (H4R3me2a) in male germ cells. (A) Detection of asymmetric dimethylarginine (ADMA) (yellow) mark in wildtype and Prmt1 KO testes on P10 and P35 by immunofluorescence. DNA was stained with 7′-AAD (blue). Scale bar, 50 μm. (B) Western blot analysis to check total ADMA in total protein from wildtype and Prmt1 KO testes on P8, P10, and P14. GAPDH was used as an endogenous control. (C) Detection of H4R3me2a (green) expression in wildtype and Prmt1 KO testes on P10 and P35 by immunofluorescence. DNA was stained with 7′-AAD (red). Scale bar, 50 μm. (D) Western blotting analysis to check H4R3me2a of total protein from wildtype and Prmt1 KO testes at P20. GAPDH was used as an endogenous control.