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. 2021 Jul 26;22(15):7943. doi: 10.3390/ijms22157943

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Deletion of DSK2s results in asynchronous replication of T. gondii. (a) IFA detected the division of Δku80, Δdsk2a, Δdsk2b and ΔΔdsk2adsk2b. IMC1 was used to stain the daughter parasites. GAP45 was used to label the parental parasites. Scale bar: 2.5 μm. (b) IFA detected the duplication of a centrosome in Δku80 and ΔΔdsk2adsk2b. Centrin1 was used to label the centrosome. White triangle indicates the tachyzoite in which the duplication of centrosome occurs before daughter IMC formation. Arrow indicates the tachyzoite in which the duplication of centrosome is asynchronous. Scale bar: 2.5 μm. (c) IFA detected apicoplasts in Δku80 and ΔΔdsk2adsk2b. ENR was used to label apicoplasts. Arrows indicate tachyzoites with more than two apicoplasts. Scale bar: 2.5 μm.