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. 2021 Jul 26;22(15):7943. doi: 10.3390/ijms22157943

Figure 6.

Figure 6

Deletion of DSK2s inhibits T. gondii growth. (a) Replication assay of the ΔΔdsk2adsk2b and Δku80 strains. IFA was performed after growth in HFF cells for 24 h. Anti-GAP45 antibody was used to stain parasites. Tachyzoites were counted in 100 PVs. Data represents means ± SEM for two independent experiments. ** p < 0.01. (b,c) Plaque formation of ΔΔdsk2adsk2b and Δku80 strains. A total of 500 tachyzoites were inoculated in HFFs for 7 days and then stained with crystal violet (b). Quantification of plaque sizes (c). 10 plaques were quantified. *** p < 0.001. (d) Survival curve of BALB/c mice intraperitoneally injected with 100 tachyzoites of ΔΔdsk2adsk2b or Δku80 strain for 10 days. ns: not significant.