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. 2021 Jul 27;22(15):7993. doi: 10.3390/ijms22157993

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Relative gene expression in Arabidopsis DR5 (DR5rev::GFP) line upon 3 h exposure to 100 mM NaCl or 200 mM mannitol compared to the Col-0 in each of the tested conditions of: tryptophan aminotransferase related 2 (TAR2, At4g24670); flavin-binding monooxygenase family protein (YUC5, At5g43890); auxin amidohydrolase (IAR3, At1g51760); auxin amidosynthetase (GH3.1, At2g14960); auxin amidosynthetase (GH3.3, At2g23170); auxin amidosynthetase (GH3.12, At5g13320); uridine diphosphate glycosyltransferase 74E2 (UGT74E2, At1g05680); 2-oxoglutarate and Fe(II)-dependent oxygenase 2 (DAO2, At1g14120), and auxin transport proteins PIN2 (At5g57090) and PIN4 (At2g01420). Gene expression was calculated according to Livak [45] and presented as fold change compared to Col-0. Asterisk (*) indicates significant differences with respect to the Col-0 growth conditions as the control (Student’s t test, * presents p < 0.05, ** p< 0.01, *** p < 0.001). Bars show means ± SE of three biological replicates.