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. 2021 Jul 27;22(15):7996. doi: 10.3390/ijms22157996

Table 3.

The solvent gradient program for the analysis of dopamine levels.

Time (min) Solvent
A (%) B (%)
0 100 0
15 40 60
18 20 80
26 20 80
30 100 0
40 100 0

Solvent A: water and Solvent B: acetonitrile, both with 0.1% formic acid (v/v). Samples were analyzed at a constant flowrate of 0.4 mL/min.