Table 3.
The solvent gradient program for the analysis of dopamine levels.
Time (min) | Solvent | |
---|---|---|
A (%) | B (%) | |
0 | 100 | 0 |
15 | 40 | 60 |
18 | 20 | 80 |
26 | 20 | 80 |
30 | 100 | 0 |
40 | 100 | 0 |
Solvent A: water and Solvent B: acetonitrile, both with 0.1% formic acid (v/v). Samples were analyzed at a constant flowrate of 0.4 mL/min.