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. 2021 Jul 30;2(3):100709. doi: 10.1016/j.xpro.2021.100709

Figure 2.

Figure 2

WICHCR supports complex fluorophore combinations for advanced experimental design

(A) WICHCR against Sox10, barx1, sox10, pHH3 and mitfa in 3 dpf WT zebrafish larvae.

(B–F) Individual panels for each channel after spectral deconvolution. Channels were initially imaged as the following sets: 488/514 (Sox10 AB/barx1), 546/594 (sox10 HCR/pHH3), and 647 (mitfa).

(G–I) HCR probe against sox10 and antibody against Sox10 show expected overlapping expression of mRNA and protein.

(J–L) Channel deconvolution efficiently separates an immunologically and HCR labeled marker with nearby excitation/emission wavelengths. Sox10 antibody (J and K) and barx1 mRNA (J and L) are shown. M-P. WICHCR permits the detection of post-translational modifications and cell identity. Scale bar = 100 μm for A–L and 50 μm for M–P. Anterior to the right and posterior to the left for all the panels. ov = otic vesicle, e = developing eye.