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WICHCR markers and detection wavelengths for an example of 3 and 5 marker experiment
|
Figure 1 (3-marker WICHCR) |
Figure 2 (5-marker WICHCR) |
|||||
|---|---|---|---|---|---|---|
| Marker | Type and concentration | Detection wavelength | Marker | Type and concentration | Detection wavelength | |
| 1 | elavl3 | HCR (B2) | 488 | Sox10 | Ab (rabbit) (1:250) |
488 |
| 2 | phox2bb | HCR (B1) | 546 | barx1 | HCR (B3) | 514 |
| 3 | Hu C/D | Ab (mouse IgG2b) (1:250) |
647 | sox10 | HCR (B5) | 546 |
| 4 | n/a | n/a | n/a | pHH3 | Ab (mouse IgG1) (1:1000) |
594 |
| 5 | n/a | n/a | n/a | mitfa | HCR (B1) | 647 |
