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60× E3 stock solution
| Reagent | Final concentration | Amount |
|---|---|---|
| Sodium Chloride | 300 mM | 140 g |
| Calcium Chloride dihydrate | 200 mM | 23.4 g |
| Potassium Chloride (anhydrous) | 100 mM | 6.1 g |
| Magnesium sulfate heptahydrate | 2 mM | 3.9 g |
| MilliQ H2O | n/a | Bring up to 8 L |
| Total | n/a | 8 L |
