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. 2021 Jul 30;2(3):100709. doi: 10.1016/j.xpro.2021.100709

1.5× PTU/E3 Working stock solution

Reagent Final concentration Amount
25× PTU stock solution 0.3 mM 60 mL
60× E3 1× 16.67 mL
20× Methylene Blue stock solution 1× 50 μL
MilliQ H2O n/a 923.33 mL
Total n/a 1000 mL