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. 2021 Jul 30;2(3):100706. doi: 10.1016/j.xpro.2021.100706
REGENT OR SOURCE SOURCE IDENTIFIER
Chemicals, peptides, and recombinant proteins

PEI PolyScience Cat#23966
Polybrene Sigma Cat#H9265
Gelatin Sigma Cat#ES-006-B
Opti-MEM Gibco Cat#31985-023
DMEM HyClone Cat#SH30022-2B
FBS Gibco Cat#NTC-HK008
NEAA Gibco Cat#11140-050
GlutaMAX Gibco Cat#35050-061
Penicillin/Streptomycin HyClone Cat#SV30010
Confocal dish WPI Cat#FD35-100
Precoated confocal dish WPI Cat#FD35PDL-100
0.45 μm Sterile filter Millipore Cat#SLHVR33RB

Recombinant DNA

pMXs-H1-GFP This study N/A
pMXs-HP1α -Cherry This study N/A

Experimental models: Cell lines

MEF cells This study N/A
Human fibroblast This study N/A
Platinum-E (plat-E) A gift from the Fourth Military Medical University N/A

Software and algorithms

Zeiss Zen2 Zeiss Zeiss, commercially available
Fiji ImageJ Schneider et al., 2012 https://imagej.net/Fiji/Downloads
Multi measure plugin N/A https://www.optinav.info/Multi-Measure.htm
StackReg plugin Thevenaz et al., 1998 http://bigwww.epfl.ch/thevenaz/stackreg/
TurboReg plugin Thevenaz et al., 1998 http://bigwww.epfl.ch/thevenaz/turboreg/
GraphPad Prism 5.0 GraphPad Software Inc. https://www.graphpad.com/scientific-software/prism/
Microsoft Excel Microsoft Microsoft

Other

LSM880 confocal microscope with incubator system. Zeiss Zeiss, commercially available