| Chemicals, peptides, and recombinant proteins |
|
| PEI |
PolyScience |
Cat#23966 |
| Polybrene |
Sigma |
Cat#H9265 |
| Gelatin |
Sigma |
Cat#ES-006-B |
| Opti-MEM |
Gibco |
Cat#31985-023 |
| DMEM |
HyClone |
Cat#SH30022-2B |
| FBS |
Gibco |
Cat#NTC-HK008 |
| NEAA |
Gibco |
Cat#11140-050 |
| GlutaMAX |
Gibco |
Cat#35050-061 |
| Penicillin/Streptomycin |
HyClone |
Cat#SV30010 |
| Confocal dish |
WPI |
Cat#FD35-100 |
| Precoated confocal dish |
WPI |
Cat#FD35PDL-100 |
| 0.45 μm Sterile filter |
Millipore |
Cat#SLHVR33RB |
|
| Recombinant DNA |
|
| pMXs-H1-GFP |
This study |
N/A |
| pMXs-HP1α -Cherry |
This study |
N/A |
|
| Experimental models: Cell lines |
|
| MEF cells |
This study |
N/A |
| Human fibroblast |
This study |
N/A |
| Platinum-E (plat-E) |
A gift from the Fourth Military Medical University |
N/A |
|
| Software and algorithms |
|
| Zeiss Zen2 |
Zeiss |
Zeiss, commercially available |
| Fiji ImageJ |
Schneider et al., 2012 |
https://imagej.net/Fiji/Downloads |
| Multi measure plugin |
N/A |
https://www.optinav.info/Multi-Measure.htm |
| StackReg plugin |
Thevenaz et al., 1998 |
http://bigwww.epfl.ch/thevenaz/stackreg/ |
| TurboReg plugin |
Thevenaz et al., 1998 |
http://bigwww.epfl.ch/thevenaz/turboreg/ |
| GraphPad Prism 5.0 |
GraphPad Software Inc. |
https://www.graphpad.com/scientific-software/prism/ |
| Microsoft Excel |
Microsoft |
Microsoft |
|
| Other |
|
| LSM880 confocal microscope with incubator system. |
Zeiss |
Zeiss, commercially available |