Stock solutions of mitochondrial substrates and cofactors
| Reagent | Final concentration | Amount |
|---|---|---|
| ADP | 0.5M, pH 7.2 (with KOH) | 1 g in 3.99 mL (final volume) TC-grade H2O |
| BSA | 10% (w/v) | 1 g in 10 mL (final volume) TC-grade H2O |
| Pyruvate | 0.5M, pH 7.2 (with KOH) | 1 g in 22.71 mL (final volume) TC-grade H2O |
| Malate | 0.5M, pH 7.2 (with KOH) | 1 g in 14.92 mL (final volume) TC-grade H2O |
| Glutamate | 0.5M, pH 7.2 (with KOH) | 1 g in 13.59 mL (final volume) TC-grade H2O |
| Palmitoyl CoA | 10 mM | 10 mg in 1 mL TC-grade H2O |
| Carnitine | 0.5M, pH 7.2 (with KOH) | 1 g in 12.41 mL (final volume) TC-grade H2O |
| Palmitoyl carnitine | 10 mM | 10 mg in 2.50 mL 95% EtOH |
| Succinate | 0.5M, pH 7.2 (with KOH) | 1 g in 16.94 mL (final volume) TC-grade H2O |
| Glycerol-3-phosphate | 0.25M, pH 7.2 (with KOH) | 1 g in 10.80 mL (final volume) TC-grade H2O |
| Ascorbate | 1M, pH 7.2 (with KOH) | 1 g in 5.68 mL (final volume) TC-grade H2O |
| TMPD | 10 mM | 1 g in 4.22 mL (final volume) TC-grade H2O |