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HepG2 Culture Medium (can be made prior to the assay and stored at 4°C for 1 month)
| Reagent | Final concentration | Amount |
|---|---|---|
| GlutaMAX | 1:100 | 5 mL |
| Penicillin/Streptomycin | 1:100 | 5 mL |
| Fetal Bovine Serum | 10% | 50 mL |
| Minimum Essential Medium | n/a | 440 mL |
| Total | n/a | 500 mL |
