MSHE without BSA (can be made and stored ahead of time at 4°C for 3 months)

Reagent	Final concentration	Amount
Mannitol	210 mM	19.13 g
Sucrose	70 mM	11.98 g
HEPES (1M stock solution)	5 mM	2.5 mL
EGTA (250 mM stock solution; pH to 8.0 with KOH)	1 mM	2 mL
KOH (1 and 10M stock solutions)	n/a	to pH 7.2
Tissue culture grade water	n/a	fill to 500 mL
Total	n/a	500 mL