| Port | Stock solution | Final concentration (mM or μM) | Media volume | Effector volume to add |
|---|---|---|---|---|
| A | Oligomycin (6.3 mM) | 2.25 μM | 3 mL | 7.5 μL |
| B & C | FCCP (10 mM) | 1.5 μM | 6 mL | 7.2 μL |
| D | Rotenone (2 mM) | 0.2 μM | 3 mL | 3 μL |
| Antimycin-A (10 mM) | 1 μM | 3 μL |
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