Four key enzyme activities of JA synthesis pathway in D52S, NK58S and PA64S spikelets by photoperiod and temperature treatment. The LD/SD and HT/LT treated plants are columnar with blue/yellow and red/blue color, respectively. Data are shown as mean ± standard deviation (n = 3). Asterisks indicate significant differences between sterile and fertile plants revealed by Student’s t-test (**, p < 0.01). LD, long-day cycle; SD, short-day cycle; HT, high temperature; LT, low temperature; S/F, sterile/fertile plants; 5/6/7, 5th/6th/7th-stage; LOX, lipoxygenases; AOS, allene oxide synthase; AOC, allene oxide cyclase; OPR, oxophytodeinoate reductase.